John Owen was an English nonconformist Church leader who served as the academic administrator at the University of Oxford. Owen was also a prolific writer. In the book A Display of Arminianism Owen discusses the differences between Calvinism and Arminianism.
Take stress out of the holidays and put fun into them, with your own, hand-colored ornaments.This black-and-white coloring book features the same bold lines and big, easy spaces of Kara & Aisling's "Bold and Easy" coloring books, and uses those designs in ornaments.This is the second (of three) books featuring bold and easy ornaments to delight colorists from age 3 to 103.Every page is printed on one side. Every page features three similar ornaments, in different sizes.35 different designs. 35 different, single-sided pages. Every ornament is round (circle shapes), and printed in black & white for easy coloring.Bonus: five pages of how-to ideas for making the most of these ornaments, and lots of bonus coloring pages for more fun.Color ornaments with your family, as a project you'll remember -- and ornaments you'll enjoy -- for many years.Also ideal for classrooms, Scouts, and art therapy of all kinds.These Christmas ornaments make it fun and easy to color a happier, stress-free holiday season.
Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display - A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. An introductory chapter provides an overview of phage biology and phage display, including guidelines for planning a successful phage display experiment. Individual chapters provide protocols for constructing libraries using oligonucleotide-directed mutagenesis or DNA recombination, performing binding selections, and analyzing the binding activities of selected phage clones. Separate chapters then cover common applications, including selection of ligands from peptide libraries, generation of phage antibody libraries and isolation and optimization of antibodies, selection of DNA binding proteins, and expression cloning using cDNA display. Further chapters describe alternative selection strategies, such as selection using immune sera, selection based on enzymatic activity or protein stability, and selection in vivo. Protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples given to customize the procedures to their own protein or selection system of interest. Written by experts in the field, Phage Display - A Practical Approach provides a comprehensive guide to the design and execution of phage display projects, for all those using the technique in basic research and drug discovery.